Combined effects of calcium and dibutyryl cyclic AMP on germinal vesicle breakdown in the mouse oocyte
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منابع مشابه
Combined effects of calcium and dibutyryl cyclic AMP on germinal vesicle breakdown in the mouse oocyte.
Mouse oocytes were cultured in the presence of dibutyryl cyclic AMP (dbcAMP) and various agents that affect cytoplasmic calcium concentrations. Treatment that inhibited calcium uptake potentiated the inhibitory effect of dbcAMP and treatments which stimulated cellular calcium uptake overcame the effect of dbcAMP. Elevated extracellular calcium (greater than 10 mM) significantly decreased the in...
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Germinal vesicle breakdown in mouse oocytes in vivo and in vitro has been examined by electron microscopy. In vitro oocytes were studied immediately after release from follicles and at various times (05-11 h) in culture. Approximately 30 min after oocyte release, chromatin condensation begins along the convoluted nuclear envelope (NE). Chromatin granules are common in all condensing chromosomes...
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Intact germinal vesicle (GV) arrest and release are essential for maintaining the fertility of mammals inducing human. Intact germinal vesicle release, maturation of oocytes is maintained by very complex procedures along with folliculogenesis and is a critical step for embryonic development. Cyclic guanosine monophosphate (cGMP) has been suggested a key factor for meiotic arrest but so far its ...
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ZP3 is a principal component of the zona pellucida (ZP) of mammalian oocytes and is essential for normal fertility, and knockout of ZP3 causes complete infertility. ZP3 promotes fertilization by recognizing sperm binding and activating the acrosome reaction; however, additional cellular roles for ZP3 in mammalian oocytes have not been yet reported. In the current study, we found that ZP3 was st...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1982
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0660001